terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Antimicrobial activity of oenological polyphenols against Gram positive and Gram negative intestinal multidrug-resistant bacteria

Antimicrobial activity of oenological polyphenols against Gram positive and Gram negative intestinal multidrug-resistant bacteria

Abstract

Bacterial antibiotic resistance is a major current health problem. Polyphenols have demonstrated antibacterial activity, and in this work we studied the effect of oenological polyphenols on the growth of intestinal multidrug-resistant strains of human and animal origin. Two Enterococcus faecium strains, resistant to vancomycin and other antibiotics, and four Escherichia coli strains, resistant to ampicillin and other antibiotics, were included in this study. All strains showed multidrug resistant phenotypes and genotypes to at least two antibiotic families. Two Vitis vinifera extracts obtained from red grape skins (GG) and seeds (TS) were studied. Standards of malvidin, epicatechin and myricetin were also included in this study. The antimicrobial activities of the polyphenolic extracts and standards alone and in combination with the corresponding antibiotic of reference were evaluated against the six multidrug-resistant strains. Minimal inhibitory concentration (MIC) and fractional inhibitory concentration index (FIC) were determined. FIC values were interpreted as follows: synergy (FIC≤0.5); partial synergy (0.5<FIC<1); additive effect (FIC=1); indifference (1<FIC<2) and antagonism (FIC≥2).

The oenological extracts tested alone inhibited the growth of the six multidrug-resistant strains: GG (MIC=6.25 mg/mL) and TS (MIC≥1 mg/mL), and their effect was bacteriostatic. Combined with the corresponding antibiotic, GG showed a synergistic effect against all the E. coli and E. faecium strains (FICs=0.4-0.6), and it was able to reduce 3-8-fold the antibiotic MICs. Similarly, malvidin inhibited the growth of all the strains (MICs=0.67-1.34 mg/mL), it showed a synergistic effect in combination with the corresponding antibiotic against all the studied strains (FICs=0.6-0.9) and it was able to reduce 2-4-fold the antibiotic MICs. TS, epicatechin and myricetin were also able to inhibit the growth of all the strains (MICs=0.3-2.68 mg/mL) and their effect in combination with the corresponding antibiotic was either additive or indifferent (1£FICs<2).

Acknowledgements: ADER2019-I-IDD-00048 of the C.A.R./FEDER; AFIANZA 2022, PR-10-20 and PR-11-19 of the C.A.R.

DOI:

Publication date: 16/10/2023

Issue: ICGWS 2023

Type: Poster

Authors

Rocío Fernández-Pérez*, Carmen Tenorio Rodríguez and Fernanda Ruiz-Larrea
Universidad de La Rioja, ICVV (Instituto de Ciencias de la Vid y del Vino: CSIC, Universidad de La Rioja, Gobierno de La Rioja), Av. Madre de Dios 53, 26006 Logroño, Spain

Contact the author*

Keywords

antibiotic resistance, MIC, FIC, synergy, polyphenols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Physico-chemical properties of vine pruning residues with potential as enological additive

Grapes are one of the world’s primary fruit crops, and pruning activities generate high amounts of annual wood wastes [1]. These pruning shoots contain valuable phenolic compounds and could have numerous potential applications [1,2]. Consequently, the aim of this work was to evaluate the physico-chemical properties of vine pruning residues with potential as enological additives. For this purpose, grapevine shoots from 12 varieties grown in Chile were collected during the winter of 2021.

New food trend ahead? Highlighting the nutritional benefits of grapevine leaves

The wine industry produces an enormous amount of waste every year. A wider inclusion of disregarded by-products in the human diet or its use as a source of bioactive compounds is a good strategy for reducing waste. It will not only introduce an added value to a waste product but also come upon the European Union and United Nations’ demands towards more sustainable agricultural approaches and circular economy.

Predicting provenance and grapevine cultivar implementing machine learning on vineyard soil microbiome data: implications in grapevine breeding

The plant rhizosphere microbial communities are an essential component of plant microbiota, which is crucial for sustaining the production of healthy crops. The main drivers of the composition of such communities are the growing environment and the planted genotype. Recent viticulture studies focus on understanding the effects of these factors on soil microbial composition since microbial biodiversity is an important determinant of plant phenotype, and of wine’s organoleptic properties. Microbial biodiversity of different wine regions, for instance, is an important determinant of wine terroir.

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region.

Defoliation combined with exogenous ABA application results in slower ripening and improved anthocyanin profile

Reducing sugar accumulation in grape (Vitis vinifera L.) berries may be a way to mitigate the effect of climate change. Managing canopy and crop load is an effective way to do so, however, reducing canopy size has been demonstrated to induce undesirable effects on anthocyanins. The aim of this study was to test if an application of exogenous ABA on the grape berries of defoliated vines (⅔ of the leaves removed) can result in slower sugar accumulation while maintaining grape and wine quality. An experiment with defoliation and exogenous ABA application on directly on clusters (factorial design 2×2) was performed with ‘Tempranillo’ fruit-bearing cuttings.